Our techniques

Due to the wide experience of the HIV HGM BioBank staff and to the services available in cooperation with the HGUGM’s Molecular Immunobiology Laboratory, we can offer a broad list of general consultancy services and consultancy services on advanced molecular and cellular techniques.

Cellular techniques

  • Extraction, purification and cryopreservation of mononuclear cells from peripheral blood (PBMC)
  • Obtaining cellular subpopulations (lymphocytes TCD4+, lymphocytes TCD8+, monocytes, dendritic cells, Treg…)
  • Growth of cell lines without mycoplasma
  • Immortalization of B-lymphocytes
  • Obtaining explants
  • Flow cytometry
  • ELISPOT
  • Study of cytokines and chemokines
  • Other personalised studies

Molecular techniques

  • DNA extraction from peripheral blood, PBSC, cellular subpopulations, cell lines…
  • DNA/RNA/protein extraction from tissue (frozen and paraffin-embedded)
  • Amplification of genomic DNA
  • RNA extraction

Histological techniques

  • Processing of frozen and paraffin-embedded tissues
  • Histological staining

To request a custom-tailored quote, please contact HIV HGM BioBank staff directly by calling (+34) 91 529 05 29 or sending an e-mail to BioBancoVIH.hgugm@gmail.com